BioBuilder - Helion

ebook

Autor: Natalie Kuldell, Rachel Bernstein, Karen Ingram
ISBN: 978-14-919-0753-5
stron: 238, Format: ebook
Data wydania: 2015-06-22
Księgarnia: Helion

Cena książki: 152,15 zł (poprzednio: 176,92 zł)
Oszczędzasz: 14% (-24,77 zł)

Osoby, które kupiły tę książkę, wybierały także »

Today’s synthetic biologists are in the early stages of engineering living cells to help treat diseases, sense toxic compounds in the environment, and produce valuable drugs. With this manual, you can be part of it. Based on the BioBuilder curriculum, this valuable book provides open-access, modular, hands-on lessons in synthetic biology for secondary and post-secondary classrooms and laboratories. It also serves as an introduction to the field for science and engineering enthusiasts.

Developed at MIT in collaboration with award-winning high school teachers, BioBuilder teaches the foundational ideas of the emerging synthetic biology field, as well as key aspects of biological engineering that researchers are exploring in labs throughout the world. These lessons will empower teachers and students to explore and be part of solving persistent real-world challenges.

Learn the fundamentals of biodesign and DNA engineering
Explore important ethical issues raised by examples of synthetic biology
Investigate the BioBuilder labs that probe the design-build-test cycle
Test synthetic living systems designed and built by engineers
Measure several variants of an enzyme-generating genetic circuit
Model "bacterial photography" that changes a strain’s light sensitivity
Build living systems to produce purple or green pigment
Optimize baker’s yeast to produce ?-carotene

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Spis treści

BioBuilder eBook -- spis treści

Preface
- Who Should Read This Book
- Why We Wrote This Book
- A Word on Synthetic Biology Today
- Navigating This Book
- Online Resources
- Safari Books Online
- How to Contact Us
- Acknowledgments
1. Fundamentals of Synthetic Biology
- What Is Synthetic Biology?
  - Why Synthetic Biology?
  - Synthetic Biology in Context
- Introduction to Engineering and Design
  - Traditional Engineering Solutions
  - Engineering Toolkits
- The Synthetic Biology Toolkit
  - The Molecular Biology Toolkit
  - The Toolkit Expanded for Synthetic Biology
    - DNA synthesis
    - Standardization
    - Abstraction
- Wrap-Up
- Additional Reading and Resources
2. Fundamentals of Biodesign
- Illustrating a Top-Down Design Approach
- From Planning Vacations to Biodesign
  - From Design to Implementation
- Biodesign Process Overview
  - Identify an Area and Challenge
  - Brainstorm Solutions to the Challenge
  - Decide on an Approach
  - Specify Your System with Devices, Parts, and DNA
    - System-level design
    - Device-level design
    - Logic devices
    - Parts-level design
- Whats Next?
- Additional Reading and Resources
3. Fundamentals of DNA Engineering
- Framing the Discussion
- Standardization of Parts and Measurements
  - What Is Standardization?
  - Competing Standards
  - How Standards Are Established
- DNA Engineering in Practice
  - DNA Assembly Basics
  - Applying the Basics of DNA Assembly
    - Building DNA: traditional molecular cloning
    - Building DNA: BioBrick assembly
    - Building DNA: Gibson assembly
- Whats Next?
- Additional Reading and Resources
4. Fundamentals of Bioethics
- What Makes Good Work?
- Regulating for Ethical Research
  - Scientific Advances Raise Ethical Questions
  - Public Response
    - Risk assessment: minimizing harm while maximizing good
    - Self-regulation versus government legislation: freedom, fairness, and justice
    - Apprehension: respect for individuals
- Three Synthetic Biology Case Studies
  - Grow-Your-Own Glowing Plants
  - Creating Genomes
  - Biosynthetic Drugs and the Economics of Public Benefit
- Group Bioethics Activities
  - Bioethics Involves a Decision-Making Process
    - Identifying the ethical question
    - Identifying the stakeholders
    - Connecting facts with guiding ethical principles
    - The Cambridge City Council hearings: bioethics in action
  - Stakeholder Activity
- Additional Reading and Resources
5. Introduction to the BioBuilder Labs
- Biological Engineering with Synthetic Biologys Toolkit
6. Eau That Smell
- Inspiration from the Eau dcoli iGEM Project
  - Identify a Challenge
  - Brainstorm Solutions
  - System-Level Design
  - Device-Level Design
  - System-Level Design, Revised
  - Device-Level Design, Revised
  - Parts-Level Design
  - Final Device and System-Level Edits
- Additional Reading and Resources
- Eau That Smell Lab
  - Design Choices
  - Experimental Question
  - Getting Started
    - Advanced preparation
      - Prepare banana smell standards
      - Prepare turbidity standards
    - Pre-lab procedures
      - Day 1: streak out strains from stabs to plates
      - Day 2: grow liquid overnights of bacterial strains
  - Lab Protocol
    - Data collection: turbidity using a spectrophotometer
    - Data collection: turbidity using turbidity standards
    - Data collection: banana smell
7. iTune Device
- Modularity
- Insulation
- Principles of Measurement
  - Whats Normally Measured
  - Making and Reporting Measurements for Synthetic Biology
- Foundational Concepts for the iTune Device Lab
  - Promoters and RBSs
  - The Lac Operon
- Additional Reading and Resources
- iTune Device Lab
  - Design Choices
  - Experimental Question
  - Getting Started
    - Advanced preparation
      - Prepare turbidity standards
    - Pre-lab procedures
      - Day 1: streak out strains from stabs to plates
      - Day 2: grow liquid overnights of bacterial strains
  - Lab Protocol
    - Data collection: turbidity and color using a spectrophotometer
8. Picture This
- Introduction to Modeling
  - Computational Modeling
  - Physical Modeling
- Inspiration from the Coliroid iGEM Project
  - Device-Level Design
  - Parts-Level Design
- Additional Reading and Resources
- Picture This Lab
  - Design Choices
  - Bacterial Photographs
  - TinkerCell Modeling
  - Electronic Circuit Modeling
    - Electronics modeling activity
9. What a Colorful World
- Introduction to Chassis
  - Utility, and How to Engineer It
  - Safety, and How to Engineer It
- Background on the E. chromi iGEM Project
  - About Their Devices
  - Parts and Devices
- Additional Reading and Resources
- What a Colorful World Lab
  - Design Choices
  - Experimental Question
  - Getting Started
  - Lab Protocol
10. Golden Bread
- Engineering Reliability
  - Scheduled Maintenance
  - Redundancy
  - Building Robust Systems
- Background on the VitaYeast iGEM Project
  - Motivation
  - Parts-Level and Device-Level Design
- Additional Reading and Resources
- Golden Bread Lab
  - Design Choices
  - Experimental Question
  - Getting Started
  - Lab Protocol
  - Transforming VitaYeast
A. Laboratory Reagents and Materials
- Equipment
- Reagent Preparation and Storage
  - Solid and Liquid Media
  - Stock Solutions
  - Buffers
  - Miscellaneous
Glossary
Index